CST, 2911S, Phospho-Catenin delta-1 (Tyr228) Antibody

Polyclonal Antibody for studying Catenin-delta (Tyr228) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Catenin delta-1 (Tyr228) Antibody (CST #2911) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Catenin delta-1 (Tyr228) Antibody detects endogenous levels of catenin δ-1 protein only when phosphorylated at Tyr228. This antibody might cross react with another overexpressed phospho-tyrosine protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr228 of human/mouse catenin δ-1. Antibodies are purified by peptide affinity chromatography. Background Catenin δ-1 (p120 catenin) has an amino-terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten linked 42-amino acid repeats. The carboxy-terminal tail has no known function (1). Catenin δ-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E-cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2). Catenin δ-1 has both positive and negative effects on cadherin-mediated adhesion (3). Actin dynamics are also regulated by catenin δ-1, which modulates RhoA, Rac, and cdc42 proteins (1). Analogous to β-catenin, catenin δ-1 translocates to the nucleus, although its role at this location is unclear. Many studies show that catenin δ-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin δ-1 may function as a tumor suppressor (4). Catenin δ-1 is phosphorylated at multiple tyrosine sites along its sequence both and (5). High levels of catenin δ-1 phosphorylated at Tyr228 are commonly seen in several carcinoma cell lines. EGFR signaling induces catenin δ-1 phosphorylation at Tyr228, with the phosphorylated protein becoming localized at adherens junctions although phosphorylation is not essential in junction formation (6). Alternate Names BCDS2; Cadherin-associated Src substrate; CAS; catenin (cadherin-associated protein), delta 1; catenin delta 1; Catenin delta-1; CTND1; CTNND; CTNND1; KIAA0384; p120; p120 catenin; p120(cas); p120(ctn); P120CAS; P120CTN Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 95, 100 SOURCE: Rabbit