CST, 29135S, PME-1 (8A6-F8) Mouse Monoclonal Antibody

Monoclonal Antibody for studying PPME1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PME-1 (8A6-F8) Mouse Monoclonal Antibody (CST #29135) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PME-1 (8A6-F8) Mouse Monoclonal Antibody recognizes endogenous levels of total PME-1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant full-length mouse PME-1 protein. Background Protein phosphatase methylesterase 1 (PME-1) is an evolutionarily conserved enzyme that demethylates phosphatases (1). Post-translational modification (PTMs) of proteins is a cellular mechanism that increases the functional diversity of the proteome. Several forms of PTMs exist, including methylation and phosphorylation, the covalent addition of a methyl or phosphate group, respectively, to specific amino acids within a protein. In addition to enzymes that catalyze the addition of methyl groups or phosphates to proteins, specific enzymes that remove PTMs exist to provide an additional level of cellular regulation; methyl and phosphate PTMs are removed by methylesterases and phosphatases, respectively. Phosphoprotein phosphatase 2a (PP2A) is an essential serine/threonine phosphatase that, as part of various signal transduction pathways, regulates many fundamental cellular processes, including DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis, and development (2-4). PP2A function is regulated, in part, by phospho- and methyl modification of its catalytic subunit. PP2A is methylated at the carboxyl group of the C-terminal Leucine 309 residue by leucine carboxyl methyltransferase (LCMT). Methylation of PP2A alters its cellular localization and its ability to interact with its regulatory subunits and substrates (5-8). PP2A is demethylated by PME-1 (9,10). PME-1 KO mice are post-natal lethal, and KO tissue exhibit altered PP2A activity and phospho-proteomic profile, consistent with a critical role PME-1 plays in regulating PP2A function (11). Dysregulated PP2A activity is linked to several diseases, including certain cancers and neurodegenerative diseases like Alzheimer's disease, suggesting that PME-1 could be the target of therapeutic intervention (12-14). Alternate Names ABDH19; FLJ22226; PME-1; PME1; PPME1; Protein phosphatase methylesterase 1; protein phosphatase methylesterase-1; testicular secretory protein Li 39 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Mouse IgG1