CST, 29164SF, ZNF217 (E6X4R) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ZNF217. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, ZNF217 (E6X4R) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #29164) is ready to ship. Product Usage Information This product is the carrier free version of product #82306. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 82306 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity ZNF217 (E6X4R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total ZNF217 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ZNF217 protein. Background Zinc finger protein 217 (ZNF217) is an oncogenic transcription factor that regulates the expression of a wide variety of genes. ZNF217 is a member of the Kruppel-like factor (KLF) family of transcription factors, which consists of zinc finger proteins that bind to CACCC or GT box DNA elements (1). ZNF217 regulates the expression of its target genes through direct DNA binding, and through interaction with transcriptional co-repressors in the CoREST-HDAC complex (2). Although it was initially described as a transcriptional repressor, ZNF217 may also act as a transcriptional activator depending on the target gene (3). Amplification of the gene itself has been observed in a variety of tumors, where it is typically associated with poor prognosis (4-7). Overexpression of ZNF217 protein leads to increased cell proliferation and promotes tumor growth in breast and ovarian cancer (8,9). ZNF217 also interferes with apoptotic signaling and confers resistance to chemotherapeutic drugs in breast cancer (9,10). Alternate Names ZABC1; Zinc finger protein 217; ZN217; ZNF217 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG