CST, 2916S, CDK7 (MO1) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Cdk7. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CDK7 (MO1) Mouse Monoclonal Antibody (CST #2916) is ready to ship. Product Usage Information Western Blotting: 1:2000 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:500 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity CDK7 (MO1) Mouse Monoclonal Antibody detects endogenous levels of total CDK7 protein. This antibody does not cross-react with other CDKs. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human CDK7. The epitope corresponds to a region surrounding Gln320 of human CDK7. Background CDK-activating kinase (CAK) is a complex of CDK7 and cyclin H. The complex is involved in cell cycle regulation by phosphorylating an activating residue in the T-loop domain of cdks (1). Regulation of CAK activity is mediated by T-loop phosphorylation and by association with MAT1, both of which enhance its kinase activity toward the CTD of RNA polymerase II (2,3) and other substrates such as p53 (4). CAK is an essential component of the transcription complex TFIIH and may interact directly with TFIIH helicases (5). Alternate Names 39 kDa protein kinase; CAK; CAK1; CDK-activating kinase; CDK-activating kinase 1; CDK7; CDKN7; Cell division protein kinase 7; cyclin dependent kinase 7; Cyclin-dependent kinase 7; cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase); HCAK; homolog of Xenopus MO15 Cdk-activating kinase; kinase subunit of CAK; MO15; p39 Mo15; p39MO15; serine/threonine kinase stk1; serine/threonine protein kinase 1; serine/threonine protein kinase MO15; Serine/threonine-protein kinase 1; STK1; TFIIH basal transcription factor complex kinase subunit Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Mouse IgG2b