CST, 29176SF, CD68 (E3O7V) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD68 mouse. Validated for WB,IHC,IHC,IF,IF,F,F. Highly specific and rigorously validated in-house, CD68 (E3O7V) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #29176) is ready to ship. Product Usage Information This product is the carrier free version of product #97778. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 97778 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD68 (E3O7V) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD68 protein. Staining of uncertain specificity in mouse endometrial epithelium has been observed by immunohistochemistry. Species Reactivity: Mouse, Rat, Hamster Source / Purification Monoclonal antibody is produced by immunizing animals with mouse CD68 recombinant protein and reacts with an epitope surrounding Gly113. Background CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (1,2). It is found on the plasma membrane, as well as endosomal and lysosomal membranes (1-3). It is proposed to bind OxLDL and has been observed as a homodimer (3,4). Alternate Names Cd68; CD68 antigen; gp11; gp110; Lamp4; Macrosialin; Sca; Scard1 Specification REACTIVITY: M R Hm SENSITIVITY: Endogenous MW (kDa): 70-80, 130-140, 200 Source/Isotype: Rabbit IgG