CST, 2928S, DAPK3/ZIPK Antibody

Polyclonal Antibody for studying DAPK3. Validated for Western Blotting. Highly specific and rigorously validated in-house, DAPK3/ZIPK Antibody (CST #2928) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DAPK3/ZIPK Antibody detects endogenous levels of total DAPK3/ZIPK protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxyl terminus of human DAPK3/ZIPK. Antibodies were purified by protein A and peptide affinity chromatography. Background Death-associated protein kinase (DAPK1) is a Ca /calmodulin-regulated serine/threonine kinase that participates in a wide range of apoptotic signals including interferon-γ, tumor necrosis factor α, Fas, activated c-Myc, and detachment from the extracellular matrix. In addition to the kinase domain and calmodulin regulatory segment, DAPK1 also has eight ankyrin repeats, a cytoskeleton binding region, and a conserved death domain (1-3). Deletion of the calmodulin-regulatory domain generates a constitutively active mutant kinase. Ectopic expression of wild-type DAPK1 induced cell death in HeLa cells. Conversely, expression of a catalytically inactive mutant protected cells from interferon-γ-induced cell death (4). The catalytic domain of DAPK1 has very high sequence similarity to vertebrate myosin light chain kinase (MLCK) and a RXX(S/T)X motif derived from myosin light chain protein was shown to be phosphorylated by DAPK1 (5). The DAPK family consists of several kinases including DAPK, DAPK2/DRP-1 (6), and DAPK3/ZIPK/DLK (7-9) with homology in their catalytic domain. Overexpression of DAPK3/ZIPK, but not a catalytically inactive mutant, can induce apoptosis (7). DAPK3 was also identified as a myosin light chain kinase, demonstrating ability to phosphorylate the regulatory light chain of myosin II in a Ca /calmodulin-independent manner (8). In addition to an amino-terminal kinase domain, DAPK3 contains a carboxy-terminal leucine zipper domain that mediates interaction with leucine zipper transcription factors such as ATF4 (7). DAPK3 is predominantly localized to the nucleus and has been found in PML oncogenic domains (PODs) associated with DAXX and PAR-4, and can phosphorylate PAR-4 (10,11). In addition, DAPK3 can phosphorylate STAT3 at Ser727 to enhance its transcriptional activity (12). Alternate Names DAP kinase 3; DAP-like kinase; DAPK3; death associated protein kinase 3; Death-associated protein kinase 3; Dlk; FLJ36473; MYPT1 kinase; ZIP; ZIP kinase isoform; ZIP-kinase; ZIPK; Zipper-interacting protein kinase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 52 SOURCE: Rabbit