CST, 29302SF, Proglucagon (D16G10) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Glucagon. Validated for Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, Proglucagon (D16G10) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #29302) is ready to ship. Product Usage Information This product is the carrier free version of product #8233. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8233 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Proglucagon (D16G10) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total proglucagon protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala137 of human proglucagon protein. Background Glucose homeostasis is regulated by a variety of hormones, including glucagon. Glucagon is synthesized as the precursor molecule proglucagon and is proteolytically processed to yield the mature peptide in α cells of the pancreatic islets. Glucagon causes the release of glucose from glycogen and stimulates gluconeogenesis in the liver (1,2). Alternate Names GCG; Glicentin; Glicentin-related polypeptide; GLP-1; GLP-1(7-36); GLP-1(7-37); GLP-2; GLP1; GLP2; GLUC; Glucagon; Glucagon-like peptide 1; Glucagon-like peptide 1(7-36); Glucagon-like peptide 1(7-37); Glucagon-like peptide 2; GRPP; Incretin hormone; OXM; OXY; Oxyntomodulin; preproglucagon; Pro-glucagon Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG