CST, 2936S, Cyclin D3 (DCS22) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Cyclin D3. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Cyclin D3 (DCS22) Mouse Monoclonal Antibody (CST #2936) is ready to ship. Product Usage Information Western Blotting: 1:2000 Immunohistochemistry (Paraffin): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 99580 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Cyclin D3 (DCS22) Mouse Monoclonal Antibody detects endogenous levels of total cyclin D3 protein. This antibody does not cross-react with cyclin D1 or cyclin D2. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human cyclin D3 corresponding to residues 241-260. Background Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4). Although the D-type cyclins are not fully redundant, cyclin D3, like D1, plays a prominent role in differentiation and proliferation, which correlates with higher expression levels of cyclin D3 in various cancers (5). Alternate Names CCND3; cyclin D3; D3-type cyclin; G1/S-specific cyclin D3; G1/S-specific cyclin-D3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 31 Source/Isotype: Mouse IgG1