CST, 2940S, p58IPK (C56E7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying P58IPK. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, p58IPK (C56E7) Rabbit Monoclonal Antibody (CST #2940) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity p58IPK (C56E7) Rabbit Monoclonal Antibody detects endogenous levels of total p58IPK protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp127 of human p58IPK. Background p58IPK is an inhibitor of interferon-induced and double-stranded RNA-activated protein kinase (PKR). It physically interacts with PKR and inhibits its activation and activity (1). Influenza virus activates p58IPK and thus blocks the activity of PKR to repress translation in the infected cells. In the uninfected cells, p58IPK forms a complex with its own inhibitor, HSP40, and is kept in an inactive state (2). ER stress induces the expression of p58IPK mediated by ATF6 (3,4). The induced p58IPK negatively regulates Perk activity, inhibits eIF2α phosphorylation and suppresses the activation of expression of downstream ER-responsive genes ATF4 and GADD153 (4). More recently, p58IPK has been shown to associate with the lumen of the endoplasmic reticulum (ER) where it is believed to serve as a cochaperone for BiP (5). Alternate Names ACPHD; DnaJ (Hsp40) homolog, subfamily C, member 3; DnaJ heat shock protein family (Hsp40) member C3; DnaJ homolog subfamily C member 3; DNAJC3; DNJC3; Endoplasmic reticulum DNA J domain-containing protein 6; ER-resident protein ERdj6; ERdj6; FLJ21288; HP58; Interferon-induced, double-stranded RNA-activated protein kinase inhibitor; P58; p58(IPK); P58IPK; PRKRI; Protein kinase inhibitor of 58 kDa; Protein kinase inhibitor p58; protein-kinase, interferon-inducible double stranded RNA dependent inhibitor Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 58 Source/Isotype: Rabbit IgG