CST, 29460SF, Mast Cell Chymase/CMA1 (F6S4Q) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CMA1. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Mast Cell Chymase/CMA1 (F6S4Q) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #29460) is ready to ship. Product Usage Information This product is the carrier free version of product #18992. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 18992 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Mast Cell Chymase/CMA1 (F6S4Q) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total mast cell chymase/CMA1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr195 of human mast cell chymase/CMA1 protein. Background Mast cell chymase, also known as chymase 1 (CMA1), is one of the major proteases expressed and secreted by mast cells (1). CMA1 is found in cytoplasmic granules before it is released. It converts angiotensin I to angiotensin II and is the major enzyme responsible for doing so in the heart (2). CMA1 can promote pathogenesis of chronic obstructive pulmonary disease (COPD) by inducing TNF-α in lung macrophages (3). CMA1 activity is also implicated in fibrosis in various tissues (4). In COVID-19 patients, CMA1-positive mast cells are significantly elevated, and CMA1 levels are associated with disease severity (5,6). In gastric cancer, CMA1 has been proposed as a prognostic marker, with high expression associated with poor overall survival and with high levels of tumor-infiltrating lymphocytes (TILs) (7). Alternate Names Alpha-chymase; Chymase; chymase 1; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase 1, mast cell; chymase, heart; chymase, mast cell; CMA1; CYH; CYM; Mast cell protease I; MCT1; MGC119890; MGC119891 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG