CST, 29499S, CD48 (D7L8I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD48. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD48 (D7L8I) Rabbit Monoclonal Antibody (CST #29499) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:200 Immunohistochemistry (Paraffin): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 90615 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity CD48 (D7L8I) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD48 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CD48 protein. Background CD48 is a glycosylphosphatidylinositol (GPI) -anchored membrane protein of the signaling lymphocyte activation molecule (SLAM) family, also known as SLAMF2 and BLAST-1. It is constitutively expressed on most hematopoietic cells (not on neutrophils and a subset of long-term hematopoietic stem cells in mice) and can be upregulated under certain conditions like infection (1). Interaction with its low affinity ligand CD2 promotes adhesion and TCR signaling (2,3). Interaction with the high affinity ligand CD244 (2B4) regulates natural killer (NK) and CD8 T cell activation and cytolytic function (4-6). Alternate Names B-lymphocyte activation marker BLAST-1; BCM1; BCM1 surface antigen; BLAST; BLAST1; CD48; CD48 antigen; CD48 antigen (B-cell membrane protein); CD48 molecule; hCD48; Leukocyte antigen MEM-102; mCD48; MEM-102; Signaling lymphocytic activation molecule 2; SLAM family member 2; SLAMF2; TCT.1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45-50 Source/Isotype: Rabbit IgG