CST, 29510SF, Myogenin (E9A1S) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying MYOG. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Myogenin (E9A1S) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #29510) is ready to ship. Product Usage Information This product is the carrier free version of product #43098. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 43098 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Myogenin (E9A1S) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total myogenin protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu146 of human myogenin protein. Background Myogenin (MYOG), also called myogenic factor 4 (Myf-4), is a transcriptional regulatory protein that belongs to the myogenic basic helix-loop-helix protein family, which also includes MyoD, Myf-5, and MRF4 (1). It is involved in skeletal muscle development and differentiation and is a marker for myogenesis (1,2). Detection of aberrant myogenin expression is also useful in pathology as an indicator of rhabdomyosarcoma (RMS) (2,3). Alternate Names BHLHC3; Class C basic helix-loop-helix protein 3; Myf-4; MYF4; MYOG; Myogenic factor 4; Myogenic factor-4; Myogenin; myogenin (myogenic factor 4) Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG