CST, 29528SF, Enolase-2 (E2H9X) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Enolase2. Validated for Western Blotting,Immunohistochemistry (Paraffin),ELISA+. Highly specific and rigorously validated in-house, Enolase-2 (E2H9X) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #29528) is ready to ship. Product Usage Information This product is the carrier free version of product #24330. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 24330 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Enolase-2 (E2H9X) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Enolase-2 protein. This antibody does not cross-react with human Enolase-1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Enolase-2 protein. Background Enolase is a glycolytic enzyme that is involved in the conversion of 2-phosphoglycerate to phosphoenolpyruvate (1). Mammalian enolase has three subunits: α, β, and γ, that can form homo and heterodimers. Homodimers of γ enolase are neuronal-specific (2). Research studies have shown elevated levels of neuro-specific enolase-2 in neuroblastoma (2) and small-cell lung cancer (3,4). Alternate Names 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolyase; ENO2; ENOG; Enolase 2; enolase 2 (gamma, neuronal); epididymis secretory protein Li 279; Gamma-enolase; HEL-S-279; Neural enolase; neuron specific gamma enolase; Neuron-specific enolase; neuronal enriched enolase; neurone-specific enolase; NSE Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 47 Source/Isotype: Rabbit IgG