CST, 2953S, TCF4/TCF7L2 (L40C3) Mouse Monoclonal Antibody

Monoclonal Antibody for studying TCF4/TCF7L2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TCF4/TCF7L2 (L40C3) Mouse Monoclonal Antibody (CST #2953) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TCF4/TCF7L2 (L40C3) Mouse Monoclonal Antibody detects endogenous levels of total TCF4/TCF7L2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing aminals with a synthetic peptide corresponding to residues surrounding Glu81 of human TCF4/TCF7L2. Background LEF1 and TCF are members of the high mobility group (HMG) DNA-binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1/TCF7), TCF3/TCF7L1, and TCF4/TCF7L2 (1). LEF1 and TCF1/TCF7 were originally identified as important factors that regulate early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers, including colon cancer (4,5). Alternate Names HMG box transcription factor 4; hTCF-4; T-cell factor 4; T-cell-specific transcription factor 4; TCF-4; TCF4; TCF7L2; TF7L2; transcription factor 7 like 2; Transcription factor 7-like 2; transcription factor 7-like 2 (T-cell specific, HMG-box) Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 58, 79 Source/Isotype: Mouse IgG1