CST, 29572S, Hepcidin (E8M5B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Hepcidin. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Hepcidin (E8M5B) Rabbit Monoclonal Antibody (CST #29572) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Hepcidin (E8M5B) Rabbit Monoclonal Antibody recognizes endogenous levels of total hepcidin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln30 of human hepcidin protein. Background Hepcidin is a secreted peptide hormone that functions as a master regulator of systemic iron homeostasis (1). Hepcidin is synthesized as a preprohormone, primarily by hepatocytes; the preprohormone undergoes a series of cleavage events, culminating in the release of mature hepcidin into circulation, where it plays a critical role in regulating plasma iron levels (2). Hepcidin in circulation binds to ferroportin, a channel protein that regulates cellular iron export, and whose expression levels are post-translationally regulated via its interactions with hepcidin (3). Chronic overexpression of hepcidin leads to iron-restricted anemia in mammals, whereas hepcidin deficiency leads to disease states associated with iron overload. Hepcidin levels can thus serve as useful biomarkers for various disease states related to iron homeostasis (4-7). Alternate Names HAMP; HEPC; Hepc20; Hepc25; Hepcidin; hepcidin antimicrobial peptide; hepcidin preproprotein; Hepcidin-20; Hepcidin-25; HFE2B; LEAP-1; LEAP1; liver-expressed antimicrobial peptide; Liver-expressed antimicrobial peptide 1; P81172; PLTR; Putative liver tumor regressor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 10 Source/Isotype: Rabbit IgG