CST, 29621S, CD33 (F3S8B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD33 mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CD33 (F3S8B) Rabbit Monoclonal Antibody (CST #29621) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CD33 (F3S8B) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD33 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse CD33 protein. Background CD33, a type I transmembrane protein, is a sialic acid-binding Ig-like lectin 3 (Siglec-3) of the Ig superfamily, and human CD33 binds preferentially to alpha-2, 6-linked sialic acid. Upon binding to its ligands, CD33 transduces inhibitory signaling through the immunoreceptor tyrosine-based inhibitory motif (ITIM) in its intracellular domain, inhibiting cellular functions such as phagocytosis. In addition, CD33 is also involved in other processes, such as adhesion (1-3). Due to its exclusive expression on hematopoietic cells, particularly the myeloid lineage and their progenitors, CD33 has been actively pursued as a therapeutic target against acute myeloid leukemia (AML) (4,5). CD33 may also be involved in Alzheimer's Disease (AD) (6-8). Alternate Names Cd33; CD33 antigen; gp6; gp67; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Sigle; Siglec-3; Siglec3 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 70-80 Source/Isotype: Rabbit IgG