CST, 29623S, MUC16 Antibody

Polyclonal Antibody for studying MUC16. Validated for Western Blotting. Highly specific and rigorously validated in-house, MUC16 Antibody (CST #29623) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody Protocol Available protocols: Western Blotting Specificity / Sensitivity MUC16 Antibody recognizes endogenous levels of total MUC16 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser12185 of human MUC16 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Mucins are a family of macromolecules that line and protect the respiratory epithelium from microbes and pollutants in the local environment. Of the family members that are known to date, some are produced in a cell type and tissue-specific manner, suggesting distinct biological roles for members. Some members polymerize after secretion to form gel-like substances that coat the epithelial layer (1). MUC16 is a member of the mucin family, which are high molecular weight, O-glycosylated proteins that play an important role in forming mucous barriers (2) and are found on the apical surface of the epithelia. It contains an extracellular domain at its amino acid terminus, a large tandem repeat, and a transmembrane domain with a short cytoplasmic domain. MUC16 has been linked to lung cancer (3) and ovarian cancer (4). It provides protective, lubricating barriers against particles and infectious agents at mucosal surfaces (5). Alternate Names CA-125; CA125; CA125 ovarian cancer antigen; FLJ14303; MUC-16; MUC16; mucin 16, cell surface associated; Mucin-16; Ovarian cancer-related tumor marker CA125; Ovarian carcinoma antigen CA125 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 200-270 SOURCE: Rabbit