CST, 29665S, Involucrin (F9C3C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Involucrin. Validated for WB,WB,IP,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Involucrin (F9C3C) Rabbit Monoclonal Antibody (CST #29665) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Involucrin (F9C3C) Rabbit mAb recognizes endogenous levels of total involucrin protein. Non-specific nuclear staining was observed in human heart by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human involucrin protein. Background Involucrin is a marker of terminal keratinocyte differentiation in stratified squamous epithelia and a component of the cross-linked cornified cell envelope (CE) adjacent to the plasma membrane (1,2). The high number of glutamine-rich repeats in the amino acid sequence of involucrin serve as active sites for the cross-linking enzyme transglutaminase (1-3). Soluble involucrin can be cross-linked to itself and other members of the CE, including cystatin alpha, loricrin, elafin, desmoplakin family proteins, spr family proteins, and keratin family proteins (3). The CE can be reconstituted in 3D bioprinted artificial human skin expressing IVL, or with cultured keratinocytes in the presence of Ca and transglutaminase (4,5). While the CE is insoluble and highly resistant to detergent extraction, cross-linked involucrin can be cleaved using cyanogen bromide (6). Mice deficient in involucrin, envoplakin, and periplakin develop dry, hyperkeratotic skin with underlying structurally abnormal CEs (7). Involucrin is overexpressed in transcription factor Foxn1-/- nude mice, one of several PKC-driven differentiation abnormalities in this model (8). Frequent coexpression of PTK6/BRK and involucrin has been observed in breast cancer tissue, and these proteins can serve as predictive biomarkers for therapeutic response to vitamin D3 (9). In squamous cell carcinoma (SCC), involucrin expression is inversely correlated with retinal G protein-coupled receptor (RGR) expression, with involucrin expression higher in well-differentiated SCC lesions (10). Alternate Names INVO; Involucrin; IVL Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 135 Source/Isotype: Rabbit IgG