CST, 29710S, Blimp-1/PRDI-BF1 (C14A4) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying PRD1. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Blimp-1/PRDI-BF1 (C14A4) Rabbit Monoclonal Antibody (PE Conjugate) (CST #29710) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Blimp-1/PRDI-BF1 (C14A4) Rabbit Monoclonal Antibody (PE Conjugate) detects endogenous levels of total Blimp-1/PRDI-BF1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val90 of human PRDI-BF1 isoform 2. Background Blimp-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor that is considered a master regulator of terminal B cell development (1). The human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-β promoter and can inhibit virus-mediated IFN-β production (2). Expression of Blimp-1 is sufficient to drive terminal differentiation of BCL1 lymphoma cells into antibody secreting plasma cells, increasing the expression of the cell surface marker Syndecan-1 (1). In the B cell lineage, Blimp-1 is specifically expressed in antibody-secreting cells including activated B and plasma cells. In addition, Blimp-1 has been found during macrophage differentiation (3) and in a subset of T cells (4,5), suggesting it may play a wider role in homeostasis and differentiation (6). Mechanistically, Blimp-1 is thought to act by recruiting chromatin-modifying enzymes including histone deacetylases (7) and methyltransferases (8,9). Target genes of Blimp-1 transcriptional repression with potential roles in differentiation include c-Myc (10), CIITA (11), Pax5 (12), Spi-B, and Id3 (13). Alternate Names B-lymphocyte-induced maturation protein 1; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP-1; BLIMP1; MGC118922; MGC118923; MGC118924; MGC118925; Positive regulatory domain I-binding factor 1; PR domain 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PR-domain zinc finger protein 1; PR/SET domain 1; PRDI-BF1; PRDI-binding factor 1; PRDI-binding factor-1; PRDM1 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG