CST, 29721S, SPDEF (F9M9S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SPDEF. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, SPDEF (F9M9S) Rabbit Monoclonal Antibody (CST #29721) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP Specificity / Sensitivity SPDEF (F9M9S) Rabbit Monoclonal Antibody recognizes endogenous levels of total SPDEF protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala100 of human SPDEF protein. Background SAM pointed domain-containing Ets transcription factor (SPDEF), also known as prostate-derived Ets factor (PDEF), was originally identified as an androgen-independent regulator of prostate-specific antigen (PSA) and binds to 5'-GGAT-3' DNA sequences (1). Expressed primarily in epithelial cells, SPDEF acts as a transcriptional regulator of epithelial cell adhesion, structure, and differentiation. SPDEF is tightly regulated across a variety of cancer types and has been reported as both a tumor oncogene and tumor supressor (2,3). In luminal breast cancer, SPDEF is overexpressed and promotes GALNT7 transcription, increasing tumor cell proliferation and stemness (4). SPDEF suppresses colorectal cancer by inhibiting β-catenin and downstream expression of cyclin D1 and c-Myc (5). Methylation of SPDEF gene enhancer regions by DNA methyltransferases (DNMTs) suppresses SPDEF expression, driving the observed differences in SPDEF transcripts and protein among prostate cancer cell lines and tumor grades (6). Alternate Names bA375E1.3; PDEF; Prostate epithelium-specific Ets transcription factor; Prostate-derived Ets factor; Prostate-specific Ets; PSE; RP11-375E1__A.3; SAM pointed domain containing ets transcription factor; SAM pointed domain-containing Ets transcription factor; SPDEF Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 37-45 Source/Isotype: Rabbit IgG