CST, 2977S, NOS (pan) Antibody

Polyclonal Antibody for studying iNOS mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, NOS (pan) Antibody (CST #2977) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NOS (pan) Antibody detects endogenous levels of total eNOS, iNOS and nNOS protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Ser1000 of human iNOS. Antibodies are purified by protein A and peptide affinity chromatography. Background Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citrulline from L-arginine, oxygen, and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body, including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3). Alternate Names i-NOS; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; iNOS; Inosl; MAC-NOS; Macrophage NOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; Nitric oxide synthase, inducible; NOS type II; Nos-2; NOS-II; Nos2; Nos2a; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 130, 140, 160 SOURCE: Rabbit