CST, 29786S, NRK1 (F4F8L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NRK1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NRK1 (F4F8L) Rabbit Monoclonal Antibody (CST #29786) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NRK1 (F4F8L) Rabbit Monoclonal Antibody recognizes endogenous levels of total NRK1 protein. This antibody does not cross-react with NRK2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp48 of human NRK1 protein. Background Nicotinamide riboside (NR) is converted to NAD precursor nicotinamide mononucleotide (NMN) by nicotinamide riboside kinase 1 and 2 (NRK1 and NRK2), a function shared by yeast Nrk1 (1). Cytosolic NRK1, encoded by the gene, is expressed in most tissues and enriched in liver and kidney, while NRK2 expression is limited to muscle (2). Primary hepatocytes from NRK1 mice were used to demonstrate that NRK1 is essential for utilization of exogenous NR or NMN to produce NAD (2). Tissue-specific expression of NAD synthesis enzymes can drive cancer cell survival and treatment strategies. Cancer cells can arise in tissues with variable expression of rate-limiting enzymes for NAD synthesis, including nicotinate phosphoribosyltransferase (NAPRT) or nicotinamide phosphoribosyltransferase (NAMPT) (3). In instances where this expression is low, NRK1-driven NAD synthesis is key for cancer cell survival and may bypass inhibitors of the other NAD pathways (3). Alternate Names bA235O14.2; C9orf95; chromosome 9 open reading frame 95; FLJ20559; Nicotinamide riboside kinase 1; Nicotinic acid riboside kinase 1; NmR-K 1; NMRK1; NRK 1; NRK1; Ribosylnicotinamide kinase 1; Ribosylnicotinic acid kinase 1; RNK 1; RP11-235O14.2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 23 Source/Isotype: Rabbit IgG