CST, 2979S, Phospho-SRC-3 (Thr24) Antibody

Polyclonal Antibody for studying SRC-3 (Thr24) phosphate. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Phospho-SRC-3 (Thr24) Antibody (CST #2979) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Phospho-SRC-3 (Thr24) Antibody detects endogenous levels of SRC-3 protein only when phosphorylated on Thr24. This antibody does not cross-react with other family members. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to sequence surrounding Thr24 of the human SRC-3 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background There are three members of the steroid receptor co-activator (SRC) family of proteins: SRC-1 (NCoA-1), SRC-2 (TIF2/GRIP1/NCoA-2), and SRC-3 (ACTR/pCIP/RAC3/TRAM-1/AIB1). All SRC family members share significant structural homology and function to stimulate transcription mediated by nuclear hormone receptors and other transcriptional activators such as Stat3, NF-κB, E2F1, and p53 (1-4). Two SRC proteins, SRC-1 and SRC-3, function as histone acetyltransferases (5,6). In addition, all three family members can recruit other histone acetyltransferases (CBP/p300, PCAF) and histone methyltransferases (PRMT1, CARM1) to target promoters and cooperate to enhance expression of many genes (5-8). The SRC proteins play important roles in multiple physiological processes including cell proliferation, cell survival, somatic cell growth, mammary gland development, female reproductive function, and vasoprotection (9). SRC-1 and SRC-3 are conduits for kinase-mediated growth factor signaling to the estrogen receptor and other transcriptional activators. Seven SRC-1 phosphorylation sites and six SRC-3 phosphorylation sites have been identified, which are induced by steroids, cytokines, and growth factors and involve multiple kinase signaling pathways (9-11). Research has shown that all three SRC family members are associated with increased activity of nuclear receptors in breast, prostate, and ovarian carcinomas. According to the literature, SRC-3 is frequently amplified or overexpressed in a number of cancers (12), and SRC-1/PAX3 and SRC-2/MYST3 translocations are found associated with rhabdomyosarcoma and acute myeloid leukemia, respectively (13,14). Phosphorylation of Thr24 of SRC-3 proteins can be induced by stimulation with EGF. Phosphorylated SRC-3 translocates from the cytoplasm to the nucleus where it interacts with other transcription factors and steroid hormone receptors and regulates gene expression (15). Alternate Names ACTR; AIB-1; AIB1; Amplified in breast cancer 1 protein; BHLHE42; CAGH16; CBP-interacting protein; Class E basic helix-loop-helix protein 42; CTG26; KAT13B; MGC141848; NCoA-3; NCOA3; Nuclear receptor coactivator 3; pCIP; RAC-3; RAC3; Receptor-associated coactivator 3; SRC-3; SRC3; Steroid receptor coactivator protein 3; Thyroid hormone receptor activator molecule 1; TNRC14; TNRC16; TRAM-1; TRAM1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 160 SOURCE: Rabbit