CST, 2986S, CNPase Antibody

Polyclonal Antibody for studying CNPase. Validated for Western Blotting. Highly specific and rigorously validated in-house, CNPase Antibody (CST #2986) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CNPase Antibody detects endogenous levels of total CNPase protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human CNPase. Antibodies are purified by protein A and peptide affinity chromatography. Background CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase) catalyzes the hydrolysis of 2', 3'-cyclic nucleotides to produce 2'-nucleotides. The molecular function and native substrate of this nucleotide phosphodiesterase remains under investigation (1). High CNPase expression is seen in oligodendrocytes and Schwann cells as CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (2). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth (3). Typical myelination is seen in CNPase knock-out mice, but they suffer severe neurodegeneration from axonal loss and oligodendrocytes display abnormal paranodal loop structure prior to axonal degeneration. Paranodal loops typically contact the axolemma in axon-glial signaling; neurodegeneration in CNPase knock-out mice is a secondary consequence of impaired cell-cell communication (4). Alternate Names 2', 3' cyclic nucleotide 3' phosphohydrolase; 2',3'-cyclic nucleotide 3' phosphodiesterase; 2',3'-cyclic-nucleotide 3'-phosphodiesterase; CN37; CNP; CNP1; CNPase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 47 SOURCE: Rabbit