CST, 2988S, CCL5/RANTES (P20) Antibody

Polyclonal Antibody for studying CCL5. Validated for Western Blotting. Highly specific and rigorously validated in-house, CCL5/RANTES (P20) Antibody (CST #2988) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CCL5/RANTES (P20) Antibody detects endogenous levels of total human CCL5/RANTES protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro20 of human CCL5/RANTES. Antibodies were purified by protein A and peptide affinity chromatography. Background RANTES/CCL5 (regulated upon activation, T cell expressed and secreted) is a member of the C-C or β family of chemokines that induce inflammation and are associated with a number of inflammatory disorders (1,2). RANTES is produced and secreted mainly by CD8 T cells, macrophages, and platelets, as well as epithelial cells, fibroblasts, and some solid tumors (2-7). RANTES acts as a chemoattractant and has other regulatory functions on a number of cell types, including monocytes, memory T cells, NK cells, eosinophils, basophils, dendritic cells, and mast cells (3, 7-9). Signaling by RANTES is mediated by several G protein-coupled receptors (GPCRs), including CCR1, CCR3, CCR4, and CCR5. Alternate Names beta-chemokine RANTES; C-C motif chemokine 5; C-C motif chemokine ligand 5; CCL5; chemokine (C-C motif) ligand 5; D17S136E; EoCP; Eosinophil chemotactic cytokine; MGC17164; RANTES; RANTES(3-68); RANTES(4-68); regulated upon activation, normally T-expressed, and presumably secreted; SCYA5; SIS-delta; SISd; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 10 SOURCE: Rabbit