CST, 2996T, SET8 (C18B7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SET8. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, SET8 (C18B7) Rabbit Monoclonal Antibody (CST #2996) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SET8 (C18B7) Rabbit Monoclonal Antibody detects endogenous levels of total SET8 protein (both isoforms). This antibody also crossreacts with an unidentified protein at 50kDa. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human SET8 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background SET domain-containing lysine methyltransferase 8 (SET8), also known as PR/SET domain-containing protein 7 (PR/SET7), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1-3). SET8 is a single-subunit enzyme that mono-methylates histone H4 on Lys20, preferably on nucleosomal substrates (1-3). SET8 protein levels and Histone H4 Lys20 methylation are cell cycle regulated, both increasing in S phase and peaking at G2/M phase (4,5). SET8 interacts with the PCNA protein, associates with sites of active DNA synthesis, and is required for DNA replication and genome stability during S phase (5-7). Inhibition of SET8 using shRNA or siRNA results in arrest of replication forks, induction of double-stranded DNA breaks, and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle (6,7). Furthermore, SET8 methylates p53 on Lys382, down regulating the pro-apoptotic and checkpoint activation functions of p53 (8). In response to DNA damage, SET8 expression levels decrease, allowing p53 to activate checkpoints and/or apoptosis (8). Both the methylation of histone H4 Lys20 and p53 appear to be important for the functions of SET8 in S phase. Alternate Names H4-K20-HMTase KMT5A; H4-K20-HMTase SETD8; H4-K20-specific histone methyltransferase; H4K20-specific histone methyltransferase splice variant Set8b; Histone-lysine N-methyltransferase KMT5A; Histone-lysine N-methyltransferase SETD8; KMT5A; lysine (K)-specific methyltransferase 5A; lysine methyltransferase 5A; Lysine N-methyltransferase 5A; Lysine-specific methylase 5A; N-lysine methyltransferase KMT5A; N-lysine methyltransferase SETD8; PR-Set7; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; PR/SET07; PRSET7; SET domain containing (lysine methyltransferase) 8; SET domain-containing protein 8; SET07; SET8; SETD8 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 43 Source/Isotype: Rabbit IgG