CST, 2999S, HA-Tag (6E2) Mouse Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying . Validated for Western Blotting. Highly specific and rigorously validated in-house, HA-Tag (6E2) Mouse Monoclonal Antibody (HRP Conjugate) (CST #2999) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HA-Tag (6E2) Mouse Monoclonal Antibody (HRP Conjugate) detects transfected HA-tagged proteins. This antibody conjugate recognizes the HA-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA) . The antibody was conjugated to HRP under optimal conditions. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse IgG1