CST, 30020S, Angiomotin (F7W8U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying AMOT. Validated for WB,WB,IP,IHC,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Angiomotin (F7W8U) Rabbit Monoclonal Antibody (CST #30020) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:250 - 1:1000 Immunofluorescence (Frozen): 1:800 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:200 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Angiomotin (F7W8U) Rabbit Monoclonal Antibody recognizes endogenous levels of total angiomotin protein. Based on the sequence of the immunogenic peptide, this antibody is not expected to cross-react with the AMOT family members AMOTL1 or AMOTL2. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1075 of human angiomotin protein. Background Angiomotin (AMOT) is a widely expressed cell junction protein initially identified through its ability to bind angiostatin. Alternative splicing results in two isoforms of angiomotin, the full-length p130 and the amino-terminally truncated p80. These isoforms, along with angiomotin-like 1 (AMOTL1) and angiomotin-like 2 (AMOTL2), comprise the Motin protein family. Angiomotin is important in endothelial cell polarity, migration, and blood vessel formation during development, as well as in signaling through small GTPases and the Hippo/YAP pathway (reviewed in 1). Research studies have shown that angiomotin expression regulates migration and proliferation of breast cancer cells (2,3). Alternate Names AMOT; Angiomotin; angiomotin p130 isoform; angiomotin p80 isoform; KIAA1071 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 140, 80 Source/Isotype: Rabbit IgG