CST, 3007S, Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MAPKAPK-2 (Thr334) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit Monoclonal Antibody (CST #3007) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 53485 . Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit Monoclonal Antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at threonine 334. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr334 of human MAPKAPK-2. Background In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an kinase assay (2). Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK , but is not required for the activation of MAPKAPK-2 (2). Alternate Names MAP kinase-activated protein kinase 2; MAPK activated protein kinase 2; MAPK-activated protein kinase 2; MAPK2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MAPKAPK2; mitogen-activated protein kinase-activated protein kinase 2; MK-2; MK2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 49 Source/Isotype: Rabbit IgG