CST, 30089T, ARID3B (F9T7Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ARID3B. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ARID3B (F9T7Z) Rabbit Monoclonal Antibody (CST #30089) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ARID3B (F9T7Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total ARID3B protein. This antibody is predicted to detect all three isoforms of ARID3B. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala117 of human ARID3B protein. Background AT-rich interactive domain-containing protein 3B (ARID3B) is a DNA-binding protein and a member of the extensive ARID family of proteins, many of which are recognized for their roles in chromatin remodeling and epigenetic regulation. The ARID domain of ARID3B allows for recognition and binding to AT-rich DNA sequences, and this protein typically functions as a dimer for its DNA-binding activity (1-3). Studies have established a critical role for ARID3B in embryonic development, as its deletion can lead to various abnormalities and embryonic lethality in mice (4,5). Importantly, ARID3B also plays an essential role in p53-mediated apoptosis by regulating transcription of key target genes (6). ARID3B is also a regulator of many cancer stem cell genes and has also been linked to cellular transformation and tumor progression (7-9). This protein is present in various tumor tissues; however, its expression and relevance to disease progression are context-dependent. For example, ARID3B expression has been linked to the progression of breast cancer, ovarian cancer, and malignant neuroblastoma (9-12). At the same time, its overexpression is associated with better prognosis in colorectal and breast cancer patients (13,14). Alternate Names ARI3B; ARID domain-containing protein 3B; ARID3B; AT rich interactive domain 3B (BRIGHT- like); AT rich interactive domain 3B (BRIGHT-like); AT-rich interaction domain 3B; AT-rich interactive domain-containing protein 3B; BDP; Bright and dead ringer protein; Bright-like protein; DRIL2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG