CST, 3008S, DAPK1 Antibody

Polyclonal Antibody for studying DAPK1. Validated for Western Blotting. Highly specific and rigorously validated in-house, DAPK1 Antibody (CST #3008) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DAPK1 Antibody detects endogenous levels of total DAPK1 protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly903 of human DAPK1. Antibodies are purified by protein A and peptide affinity chromatography. Background Death-associated protein kinase (DAPK1) is a Ca /calmodulin-regulated serine/threonine kinase that participates in a wide range of apoptotic signals including interferon-γ, tumor necrosis factor α, Fas, activated c-Myc, and detachment from the extracellular matrix. In addition to the kinase domain and calmodulin regulatory segment, DAPK1 also has eight ankyrin repeats, a cytoskeleton binding region, and a conserved death domain (1-3). Deletion of the calmodulin-regulatory domain generates a constitutively active mutant kinase. Ectopic expression of wild-type DAPK1 induced cell death in HeLa cells. Conversely, expression of a catalytically inactive mutant protected cells from interferon-γ-induced cell death (4). The catalytic domain of DAPK1 has very high sequence similarity to vertebrate myosin light chain kinase (MLCK) and a RXX(S/T)X motif derived from myosin light chain protein was shown to be phosphorylated by DAPK1 (5). Epigenetic silencing of DAPK1 by promoter methylation has been observed in cases of chronic lymphocytic leukemia (6,7). Alternate Names DAP kinase 1; DAPK; DAPK1; death associated protein kinase 1; Death-associated protein kinase 1; DKFZp781I035; ROCO3 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 160 SOURCE: Rabbit