CST, 30124SF, Folate Receptor Alpha/FOLR1 (E8U2F) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying FOLR1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Folate Receptor Alpha/FOLR1 (E8U2F) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #30124) is ready to ship. Product Usage Information This product is the carrier free version of product #34265. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 34265 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Folate Receptor Alpha/FOLR1 (E8U2F) Mouse Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Folate Receptor Alpha/FOLR1 protein. Non-specific staining was observed in normal human brain by immunohistochemistry. This antibody is not recommended for immunohistochemical analysis of mouse tissues. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp77 of human Folate Receptor Alpha/FOLR1 protein. Background Folate Receptor Alpha (FOLR1) is a cysteine-rich cell-surface receptor that plays a critical role in the maintenance and transport of folate (vitamin B9) and reduced folic acid derivatives into cells and is essential for various cellular processes (1,2). FOLR1 binds to folate and facilitates its internalization into cells through receptor-mediated endocytosis or potocytosis and is important in cellular processes, including DNA synthesis and repair (1-3). Intracellular folate levels are critical to maintaining and supporting folate functions in its multiple coenzyme forms (4). It is primarily expressed in tissues requiring high folate levels, such as the placenta, kidneys, and certain cancer cells (5). FOLR1 has been found to be overexpressed in several types of cancer, including ovarian, lung, and breast cancer (6-8). Alternate Names Adult folate-binding protein; FBP; folate binding protein; Folate receptor 1; folate receptor 1 (adult); Folate receptor alpha; Folate receptor, adult; FOLR; FOLR1; FR-alpha; FRalpha; KB cells FBP; Ovarian tumor-associated antigen MOv18 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 38 Source/Isotype: Mouse IgG1 kappa