CST, 3014T, Insulin (C27C9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying insulin. Validated for WB,IHC,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Insulin (C27C9) Rabbit Monoclonal Antibody (CST #3014) is ready to ship. Product Usage Information Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:6400 - 1:25600 Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 56823 . Protocol Available protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Insulin (C27C9) Rabbit Monoclonal Antibody detects endogenous levels of total insulin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to Val42 of human insulin. Background The maintenance of glucose homeostasis is an essential physiological process that is regulated by hormones. An elevation in blood glucose levels during feeding stimulates insulin release from pancreatic β cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Insulin stimulates glucose uptake from blood into skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2). Alternate Names IDDM; IDDM1; IDDM2; ILPR; ins; Insulin; insulin (A chain, B chain); Insulin A chain; Insulin B chain; IRDN; MODY10; PNDM4; preproinsulin; proinsulin Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG