CST, 30160T, Topoisomerase II beta (F8P8I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TOP2B. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Topoisomerase II beta (F8P8I) Rabbit Monoclonal Antibody (CST #30160) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Topoisomerase II beta (F8P8I) Rabbit Monoclonal Antibody recognizes endogenous levels of total topoisomerase IIβ protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1587 of human topoisomerase IIβ protein. Background DNA topoisomerases I and II are nuclear enzymes; type II consists of two highly homologous isoforms: topoisomerase IIα and IIβ. These enzymes regulate the topology of DNA, maintain genomic integrity, and are essential for processes such as DNA replication, recombination, transcription, and chromosome segregation by allowing DNA strands to pass through each other (1). Topoisomerase I nicks and rejoins one strand of the duplex DNA, while topoisomerase II transiently breaks and closes double-stranded DNA (2). Topoisomerases are very susceptible to various stresses. Acidic pH or oxidative stress can convert topoisomerases to DNA-breaking nucleases, causing genomic instability and cell death. DNA-damaging topoisomerase-targeting drugs (e.g., etoposide) also convert topoisomerases to nucleases, with the enzyme usually trapped as an intermediate that is covalently bound to the 5+ end of the cleaved DNA strand(s). Research studies have shown that this intermediate leads to genomic instability and cell death. Thus, agents that target topoisomerases are highly sought-after cancer chemotherapeutic drugs (3). Moreover, an alanine to proline substitution at position 485 (A485P) of topoisomerase IIβ has been shown to impair early B cell development (4), and the catalytic activity of this protein modulates several oncogenes in human gliomas (5). Alternate Names antigen MLAA-44; DNA topoisomerase 2-beta; DNA topoisomerase II beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme; TOP2B; top2beta; TOPIIB; topo II beta; topoisomerase (DNA) II beta 180kDa; topoisomerase II beta; topoisomerase IIb; U937 associated antigen Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG