CST, 3017T, NF-kappaB2 p100/p52 (18D10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NFkB2 p100/p52. Validated for WB,WB,IHC,F,C&R,C&T. Available in 2 sizes. Highly specific and rigorously validated in-house, NF-kappaB2 p100/p52 (18D10) Rabbit Monoclonal Antibody (CST #3017) is ready to ship. Product Usage Information The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 . Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:300 - 1:1200 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 CUT&RUN: 1:50 CUT&Tag: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 43956 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized), CUT&RUN, CUT&Tag Specificity / Sensitivity NF-kappaB2 p100/p52 (18D10) Rabbit Monoclonal Antibody detects endogenous levels of both the p100 precursor and the p52 active form of NF-κB2. This antibody does not cross-react with other family members. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the amino-terminus of human NF-κB2 p100/p52. Background Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11). Alternate Names CVID10; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; LYT-10; LYT10; NF-kB2; NFkB-p100; NFKB, p52/p100 subunit; NFKB2; NFKB2/p52; nuclear factor kappa B subunit 2; nuclear factor Kappa-B, subunit 2; Nuclear factor NF-kappa-B p100 subunit; Nuclear factor NF-kappa-B p52 subunit; nuclear factor of Kappa light chain gene enhancer in B cells 2; nuclear factor of kappa light chain gene enhancer in B-cells 2; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100); Oncogene Lyt-10; p100; p49/p100; p52; transcription factor NFKB2 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 52 active form. 120 precursor. Source/Isotype: Rabbit IgG