CST, 30204SF, SOD2 (D3X8F) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SOD2. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, SOD2 (D3X8F) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #30204) is ready to ship. Product Usage Information This product is the carrier free version of product #13141. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13141 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity SOD2 (D3X8F) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total SOD2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SOD2 protein. Background Manganese superoxide dismutase (MnSOD or SOD2) is a mitochondrial detoxification enzyme that catalyzes the conversion of superoxide to hydrogen peroxide (1,2). Hydrogen peroxide is then decomposed to water by catalase, glutathione peroxidase, or peroxiredoxins (2). MnSOD/SOD2 and other enzymes involved in antioxidant defense protect cells from reactive oxygen species (ROS) (2). Calorie restriction leads to SIRT3-mediated deacetylation of MnSOD/SOD2 and the subsequent increase of its antioxidant activity (3). MnSOD/SOD2 also plays an essential role in mediating the protective effect of mTOR inhibition to reduce epithelial stem cell senescence (4). Alternate Names epididymis secretory sperm binding protein; gastric cancer-associated lncRNA 1; GClnc1; indophenoloxidase B; IPO-B; IPOB; manganese-containing superoxide dismutase; mangano-superoxide dismutase; Mn superoxide dismutase; Mn-SOD; MNSOD; MVCD6; SOD2; SODM; Superoxide dismutase [Mn], mitochondrial; superoxide dismutase 2; superoxide dismutase 2, mitochondrial Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 22 Source/Isotype: Rabbit IgG