CST, 30270T, SART3 (F8W1S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SART3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SART3 (F8W1S) Rabbit Monoclonal Antibody (CST #30270) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SART3 (F8W1S) Rabbit Monoclonal Antibody recognizes endogenous levels of total SART3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala954 of human SART3 protein. Background Squamous cell carcinoma antigen recognized by T cells 3 (SART3), also known as p110 or Tip110, is an RNA binding protein that binds to the U4/U6 small nuclear ribonucleoprotein complex during the recycling phase of the spliceosome cycle (1,2). SART3 mediates recruitment of USP4 to deubiquitinate PRPF3, leading to the disassembly of U4 and U6 components (3). Additionally, SART3 can target USP15 to regulate histone H2B deubiquitination (4). SART3 has also been shown to play a role in hematopoiesis regulation and pluripotency, where it controls mRNA splicing of OCT4 (5-7). During HIV-1 infection, SART3 binds to and activates the viral Tat protein, promoting viral replication (8,9). SART3 is highly expressed in many tumor tissues, and various peptides have been used to generate cytotoxic T lymphocytes for targeted immunotherapy (10-13). Alternate Names DSAP1; HIV-1 Tat-interacting protein of 110kDa; hSART-3; KIAA0156; MGC138188; P100; p110; p110 nuclear RNA-binding protein; p110(nrb); PRP24 homolog; RP11-13G14; SART-3; SART3; spliceosome associated factor 3, U4/U6 recycling protein; squamous cell carcinoma antigen recognized by T cells 3; Squamous cell carcinoma antigen recognized by T-cells 3; Tat-interacting protein of 110 kDa; TIP110 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 135 Source/Isotype: Rabbit IgG