CST, 30289S, CNOT1 (D3V5H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CNOT1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CNOT1 (D3V5H) Rabbit Monoclonal Antibody (CST #30289) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CNOT1 (D3V5H) Rabbit Monoclonal Antibody recognizes endogenous levels of total CNOT1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1581 of human CNOT1 protein. Background The evolutionarily conserved CCR4-NOT (CNOT) complex regulates mRNA metabolism in eukaryotic cells (1). This regulation occurs at different levels of mRNA synthesis and degradation, including transcription initiation, elongation, deadenylation, and degradation (1). Multiple components, including CNOT1, CNOT2, CNOT3, CNOT4, CNOT6, CNOT6L, CNOT7, CNOT8, CNOT9, and CNOT10 have been identified in this complex (2). In addition, subunit composition of this complex has been shown to vary among different tissues (3). Alternate Names AD-005; adrenal gland protein AD-005; CCR4-associated factor 1; CCR4-NOT transcription complex subunit 1; CCR4-NOT transcription complex, subunit 1; CDC39; CNOT1; DKFZp686E0722; DKFZp686O168; FLJ36492; FLJ90644; hNOT1; HPE12; KIAA1007; Negative regulator of transcription subunit 1 homolog; NOT1; NOT1 (negative regulator of transcription 1, yeast) homolog; NOT1H Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 250, 270 Source/Isotype: Rabbit IgG