CST, 30325S, F4/80 (D4C8V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EMR1 mouse. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, F4/80 (D4C8V) Rabbit Monoclonal Antibody (CST #30325) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 90302 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity F4/80 (D4C8V) Rabbit Monoclonal Antibody recognizes endogenous levels of total F4/80 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln260 of mouse F4/80 protein. Background F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (1-3). Expression of F4/80 has also been observed in microglia and subset populations of dendritic cells (4). Alternate Names Adgre1; Adhesion G protein-coupled receptor E1; AGRE1; Cell surface glycoprotein F4/80; DD7A5-; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly7; Ly71; lymphocyte antigen 71; TM7L; TM7LN3 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 65-250 Source/Isotype: Rabbit IgG