CST, 30327S, CXCL9/MIG (E6Z5W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CXCL9. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CXCL9/MIG (E6Z5W) Rabbit Monoclonal Antibody (CST #30327) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 37438 Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity CXCL9/MIG (E6Z5W) Rabbit Monoclonal Antibody recognizes endogenous levels of total CXCL9/MIG protein. This antibody does not cross-react with CXCL10, CXCL11, or CXCL2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human CXCL9/MIG protein. Background C-X-C motif chemokine ligand 9 (CXCL9, MIG) is a soluble chemokine expressed by multiple cell types, such as endothelial cells, monocytes, and tumor cells. The expression of CXCL9 is upregulated in response to proinflammatory cytokines, such as IFN-γ and TNF-α. (1,2). CXCL9 binds to CXCR3, a GPCR that is expressed on the surface of multiple populations of immune cells, particularly exclusively activated T lymphocytes (3). Binding of CXCL9 to CXCR3 promotes T cell chemotaxis and infiltration into sites of inflammation that form in settings such as viral infection and tumorigenesis (4-6). Research studies have demonstrated production of CXCL9 by tumor cells mediates tumor suppression through the recruitment of tumor antigen-specific T cells (7). Alternate Names C-X-C motif chemokine 9; C-X-C motif chemokine ligand 9; chemokine (C-X-C motif) ligand 9; CMK; crg-10; CXCL9; Gamma-interferon-induced monokine; Humig; MIG; monokine induced by gamma interferon; Monokine induced by interferon-gamma; SCYB9; Small-inducible cytokine B9 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 15 Source/Isotype: Rabbit IgG