CST, 3042S, MAPKAPK-2 Antibody

Polyclonal Antibody for studying MAPKAPK-2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, MAPKAPK-2 Antibody (CST #3042) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MAPKAPK-2 Antibody detects endogenous levels of total MAPKAPK-2 protein. This antibody does not cross-react with MAPKAPK-3 or -5. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal region of human MAPKAPK-2. Antibodies are purified by protein A and peptide affinity chromatography. Background In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an kinase assay (2). Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK , but is not required for the activation of MAPKAPK-2 (2). Alternate Names MAP kinase-activated protein kinase 2; MAPK activated protein kinase 2; MAPK-activated protein kinase 2; MAPK2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MAPKAPK2; mitogen-activated protein kinase-activated protein kinase 2; MK-2; MK2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 47, 49 (Phosphorylated). SOURCE: Rabbit