CST, 30501T, Syndecan 1 (IHC138) Mouse Monoclonal Antibody

Monoclonal Antibody for studying SDC1. Validated for IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Syndecan 1 (IHC138) Mouse Monoclonal Antibody (CST #30501) is ready to ship. Product Usage Information IHC Leica Bond: 1:1600 - 1:6400 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in a Tris-based buffer with 1% BSA and less than 0.1% sodium azide. Stable for 24 months when stored at 4°C. Do not aliquot the antibody. Protocol Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity Syndecan 1 (IHC138) Mouse Monoclonal Antibody recognizes endogenous levels of total Syndecan 1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a myeloma cell line. Background Syndecans are a family of type 1 transmembrane heparan sulfate proteoglycans comprising four members in mammals (SDC1-4) (1) encoded by four syndecan genes. Syndecans are involved in embryonic development, tumorigenesis, and angiogenesis (2). The extracellular domain harbors attachment sites for heparan sulfate and chondroitin sulfate chains, facilitating interaction with an array of proteins, including a plethora of growth factors. In addition, the hydrophobic C-terminal intracellular domain can interact with proteins containing a PDZ domain (2). These interactions place syndecans as important integrators of membrane signaling (3). Syndecans undergo proteolytic cleavage causing the release of their extracellular domain (shedding), converting the membrane-bound proteins into soluble molecular effectors (4). Syndecan 1 (SDC1) is a specific marker for plasmacytic differentiation in hematologic disorders (5-7). This cell surface proteoglycan is also expressed in normal epithelial cells and tissues as well as various types of cancer tissues (8-11). The extracellular shed form of syndecan 1 remains soluble or accumulates in the extracellular matrix where it binds growth factors, cytokines and other extracellular matrix proteins (12,13). This binding activates signaling of bound growth factors or cytokines, which results in enhanced tumor growth, dissemination, angiogenesis, and osteolysis (14-17). As a result, the level of syndecan 1 protein and its shed form may serve as prognostic factors for a list of malignancies (6,18,19). Syndecan 1 has recently been found to be a critical mediator of macropinocytosis in pancreatic cancer (20). Alternate Names CD138; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; SDC; SDC1; SYND1; syndecan; syndecan 1; syndecan proteoglycan 1; Syndecan-1 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1 kappa