CST, 30530SF, Granzyme F (E2U7L) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Granzyme F mouse. Validated for Western Blotting,Immunofluorescence (Frozen),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Granzyme F (E2U7L) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #30530) is ready to ship. Product Usage Information This product is the carrier free version of product #51886. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 51886 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Granzyme F (E2U7L) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Granzyme F protein. This antibody does not cross-react with other Granzyme proteins. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln197 of mouse Granzyme F protein. Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). In mice, ten family members have been identified: A, B, C, D, E, F, G, K, M, and N (2). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein (3). Granzyme F induces a novel cell death pathway consisting of externalization of phosphatidylserine (PS), nuclear condensation, mitochondrial damage, cytochrome c release, single-stranded DNA nicking, and caspase inactivation (4). CTLs from mice with reduced expression of Granzyme F and C display diminished cytotoxicity (5).  Alternate Names AKA granzyme G; C134; CCP; CCP4; Ctl; CTL serine protease 3; Ctla; Ctla-7; Ctla7; Cytotoxic cell protease 4; Cytotoxic serine protease 3; GRAF; Granzyme F; granzyme G; Gzmf; GZMH; MCSP; MCSP-3; MCSP3 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 30-40 Source/Isotype: Rabbit IgG