CST, 3053S, NTF2 (5A3) Mouse Monoclonal Antibody

Monoclonal Antibody for studying NTF2. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, NTF2 (5A3) Mouse Monoclonal Antibody (CST #3053) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity NTF2 (5A3) Mouse Monoclonal Antibody detects endogenous levels of total NTF2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with full-length recombinant human NTF2. Background The small GTPase Ran resides on both the cytosolic and nucleosolic sides of the nuclear pore complex (NPC) and regulates the import and export of various proteins to and from the nucleus. Like other small GTPases, Ran exists in either a GTP-bound or GDP-bound state. RanGTP that resides in the nucleus and promotes nuclear export, while cytosolic RanGDP promotes import. The gradient of RanGTP across the nuclear membrane allows for appropriate movement of cargo proteins across the NPC as well as maintenance of the mitotic spindle (1-3). Nuclear transport factor 2 (NTF2) regulates the subcellular distribution and function of Ran (4-5). The NTF2 homodimer facilitates the diffusion of RanGDP through NPCs via transient interactions with phenylalanine-glycine (FG) repeat domains on NPC proteins. NTF2 stabilizes the GDP-bound form of Ran until it is induced to dissociate by a nuclear factor in an ATP-dependent manor, thus allowing the guanine nucleotide exchange factor (GEF) RCC1 to mediate exchange of GDP for GTP on Ran (6-7). Alternate Names NTF-2; NTF2; Nuclear transport factor 2; NUTF2; Placental protein 15; PP15 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 14 Source/Isotype: Mouse IgG2a