CST, 30559S, CBX4 (E6L7X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CBX4. Validated for WB,IP,IF,ChIP,ChIP,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, CBX4 (E6L7X) Rabbit Monoclonal Antibody (CST #30559) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:200 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 CUT&RUN: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Chromatin IP, Chromatin IP-seq, CUT&RUN Specificity / Sensitivity CBX4 (E6L7X) Rabbit Monoclonal Antibody recognizes endogenous levels of total CBX4 protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro166 of human CBX4 protein. Background The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage Specification, cell death, and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. This histone methyl-transferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex (5). Histone H3 methylation at Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119 (6). CBX4 is a component of the PRC1 complex, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit (7,8). CBX4 itself is a SUMO E3 ligase, and its function influences EMT, DNA damage response, tumor angiogenesis, and self-renewal (9-13). Alternate Names CBX4; chromobox 4; chromobox homolog 4 (Pc class homolog, Drosophila); Chromobox protein homolog 4; chromobox-like protein 4; E3 SUMO-protein ligase CBX4; hPc2; NBP16; NS5ATP1-binding protein 16; Pc class 2 homolog; Pc2; Polycomb 2 homolog Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG