CST, 3059S, HSPB8/HSP22 Antibody

Polyclonal Antibody for studying HSPB8. Validated for Western Blotting. Highly specific and rigorously validated in-house, HSPB8/HSP22 Antibody (CST #3059) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HSPB8/HSP22 Antibody detects endogenous levels of total HSPB8/HSP22 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human HSPB8/HSP22. Antibodies are purified by protein A and peptide affinity chromatography. Background HSPB8 (HSP22) is a member of the small heat shock protein superfamily and the human protein is most closely related to HSP27. Similar to most other small HSPs (sHSPs), HSPB8 is predominantly transcribed in skeletal muscle and heart (1). In a two hybrid screen, HSPB8 interacted preferentially with a triple aspartate form of HSP27 which mimics HSP27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type HSP27 (2). HSPB8 has two binding domains (N- and C-terminal) that are specific for different binding partners, and has the ability to bind to itself and other sHSPs such as HSPB7 and HSPB2 (3). The chaperone-like activity is of great importance to the function of HSP22 in various processes including proliferation, apoptosis and macroautophagy (4). Alternate Names Alpha-crystallin C chain; CMT2L; CRYAC; DHMN2; E2-induced gene 1 protein; E2IG1; H11; heat shock 22kDa protein 8; heat shock 27kDa protein 8; Heat shock protein beta-8; heat shock protein family B (small) member 8; HMN2; HMN2A; HSP22; HSPB8; Protein kinase H11; Small stress protein-like protein HSP22 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 22 SOURCE: Rabbit