CST, 3062S, PDK1 Antibody

Polyclonal Antibody for studying PDK1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PDK1 Antibody (CST #3062) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PDK1 Antibody detects endogenous levels of total PDK1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the carboxy terminus of human PDK1. Antibodies are purified by protein A and peptide affinity chromatography. Background Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (1,2) including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (3). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis. Alternate Names 3-phosphoinositide dependent protein kinase 1; 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; hPDK1; MGC20087; MGC35290; PDK1; PDPK1; PDPK2; PDPK2P; PkB kinase like gene 1; PkB-like 1; PRO0461; protein kinase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 58 to 68 SOURCE: Rabbit