CST, 3070S, Phospho-IRS-1 (Tyr895) Antibody

Polyclonal Antibody for studying IRS-1 (Tyr896) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-IRS-1 (Tyr895) Antibody (CST #3070) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-IRS-1 (Tyr895) Antibody detects transfected levels of IRS-1 only when phosphorylated at Tyr895. This antibody may cross-react with other activated receptor tyrosine kinases (RTKs) and docking proteins. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr896 of human IRS-1. Antibodies are purified by protein A and peptide affinity chromatography. Background Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10). Phosphorylation of Tyr895 in IRS-1 provides a binding site for Grb2, which mediates the downstream signaling leading to MAP kinase activation and mitogenesis (11). Alternate Names HIRS-1; Insulin receptor substrate 1; IRS-1; IRS1 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 180 SOURCE: Rabbit