CST, 30712S, NBC1/SLC4A4 (F2F6A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SLC4A4. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, NBC1/SLC4A4 (F2F6A) Rabbit Monoclonal Antibody (CST #30712) is ready to ship. Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity NBC1/SLC4A4 (F2F6A) Rabbit mAb recognizes endogenous levels of total NBC1/SLC4A4 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NBC1/SLC4A4 protein. Background NBC1/SLC4A4 is an electrogenic sodium bicarbonate cotransporter. It mediates the coupled movement of Na and HCO ions across the basolateral membrane of cells. NBC1 regulates secretion and absorption of bicarbonate and modulates intracellular pH (1-3). There are five isoforms of NBC1 produced by alternative splicing, including the most studied isoforms, 1 and 2, which have different N termini. The N terminus of isoform 1 contains multiple consensus phosphorylation sites for various kinases, such as PKA, PKC, and CK II, and may play a regulatory role in the biological function of NBC1 (4). Interaction with carbonic anhydrase II, IV, and IX regulates the transporter activity of NBC1 (5-7). NBC1 is expressed in various tissues with the exception of isoform 2, which is mainly expressed in kidney proximal tubules (1). Isoform 1 is expressed in the pancreas and corneal endothelium, and at low levels in other tissues, including heart, skeletal muscle, liver, and prostate (4). Research studies have shown that mutations in the gene are linked to proximal renal tubular acidosis with ocular abnormalities (also known as renal tubular acidosis II). Alternate Names DKFZp781H1314; Electrogenic sodium bicarbonate cotransporter 1; hhNMC; HNBC1; KNBC; kNBC1; Na(+)/HCO3(-) cotransporter; NBC; NBC1; NBC2; NBCE1; NBCe1-A; pNBC; S4A4; SLC4A4; SLC4A5; Sodium bicarbonate cotransporter; sodium bicarbonate cotransporter 1 (sodium bicarbonate cotransporter, kidney; sodium bicarbonate cotransporter, pancreas); solute carrier family 4 (sodium bicarbonate cotransporter), member 4; Solute carrier family 4 member 4; solute carrier family 4, sodium bicarbonate cotransporter, member 4; solute carrier family 4, sodium bicarbonate cotransporter, member 4, brain type; solute carrier family 4, sodium bicarbonate cotransporter, member 5 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 135-145 Source/Isotype: Rabbit IgG