CST, 30790SF, Phospho-cdc25C (Ser216) (63F9) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Cdc25c (Ser216) phosphate. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Phospho-cdc25C (Ser216) (63F9) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #30790) is ready to ship. Product Usage Information This product is the carrier free version of product #4901. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 4901 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-cdc25C (Ser216) (63F9) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of cdc25C only when phosphorylated at Ser216. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser216 of human cdc25C. Background Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5). Alternate Names CDC25; CDC25C; cell division cycle 25 homolog C (S. pombe); cell division cycle 25C; Dual specificity phosphatase Cdc25C; M-phase inducer phosphatase 3; mitosis inducer CDC25; MPIP3; phosphotyrosine phosphatase; PPP1R60; protein phosphatase 1, regulatory subunit 60 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG